Endotoxin-Free Utrapure Plasmid Extraction Maxiprep System

The Endotoxin-Free Utrapure Plasmid Extraction Midiprep System is composed of macroporous silica–based resin with a high density of diethylaminoethyl (DEAE) group, which is developed exclusively for isolation of nucleic acids. Purification is based on the interaction between negatively charged phosphates of nucleic acid backbones and positively charged DEAE groups on the surface of the resin. An innovative endotoxin-removal reagent, E2 reagent, can effectively remove lipopolysaccharides from bacterial cell lysates by phase partition without chilling step. Simply add the E2 reagent into the clear cell lysate, and the lipopolysaccharide contaminants can be removed with the flow-through. By testing with the endotoxin LAL test kit, the sytem consistently keeps the endotoxin level less than 0.1 EU/ug DNA in the final plasmid DNA product.

Kit contains 15 preps

GMN1001_Protocol.pdf