TSH-R AB, RIA, 100 TESTS

The TSH RECEPTOR AUTO-ANTIBODIES assay is a Radioreceptor Assay (RRA) which detects autoantibodies that interfere with the binding of thyroid stimulating hormone (TSH) to TSH receptor. In the assay, TSH receptor is incubated with serum, and after a pre-incubation time125 I-labelled TSH (tracer). Autoantibodies and tracer compete for binding sites on the receptor. Consequently, the higher the autoantibody titer, the lower the 125 I TSH bound to the receptor. Following incubation the receptor-tracer complex is precipitated with polyethylene glycol (PEG). After centrifugation, the supernatant is removed and the precipitate counted. The units of TSH RECEPTOR AUTO-ANTIBODIES activity in the sample are determined from the calibration curve and expressed as units/L (U/L).

Catalog #: R-CT-100
Format: RIA
Label: I125
Size: 100 tests
Sample Type: Serum
Sample Volume: 50 µL
Controls: 2 levels
Range: 5-405 U/L
Sensitivity: 0.8 U/L
Incubation: 2.5 hours at room temperature with shaking
Shelf Life (weeks): 7